SLAF-seq技術

技術原理

?Genetic map – showing the relative position of known genes and/or genetic markers.

High-density linkage map based on genotyping-by-resequencing showing the most likely positions of QTLs for grain protein content measured by NIRS and KND methods in population.

GWAS – an observational study of a genome-wide set of genetic variants in different individuals to see if any variant is associated with a trait.

The x-axis shows the single nucleotide polymorphism (SNPs) along each chromosome; the y-axis is the –log10(P-value) for the association. Genome-wide P-values from the generalized linear model (GLM) and compressed mixed linear model (CMLM). Colored dots in (a) and (b) indicate SNPs with P-values < 1.99 9 106 (a < 0.05) in both models.

BSA – a technique used to identify?genetic markers associated with a mutant?phenotype. This allows geneticists to discover genes conferring disease resistance or susceptibility.

Δ(SNP-index) graph of MF (male fertile) and MS (male sterile) pool from the bulked segregant analysis (BSA). The X-axis represents the chromosome position. The Y-axis represents the Δ(SNP-index) value. The spots represent Δ(SNP-index) values calculated by the formula: [Δ(SNP-index)?=?SNP-index_Largest-SNP index_Smallest]. The black line corresponds to the fitted value of the Δ(SNP-index) and the red line represents the confidence value (99%).